Details

Autor(en) / Beteiligte

• Bagheri, Zohreh
• Assadi, Mostafa
• Small, Ernest
• Mehregan, Iraj

Titel

Cryptic Molecular-Geographical Divergence within Medicago minima Revealed by SSR Markers

Ist Teil von

• Iranian journal of science and technology. Transaction A, Science, 2022-02, Vol.46 (1), p.49-60

Ort / Verlag

Cham: Springer International Publishing

Erscheinungsjahr

2022

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Medicago minima (L.) Bartal. is an important rangeland forage and weed, native to Eurasia and Africa but widely distributed elsewhere. Like dozens of its nearest annual Medicago species, it is known to be highly uniform due to extreme inbreeding, and no valid infra-specific taxa meriting recognition have yet been proposed. In this study, 67 individuals from 13 natural populations of M. minima were collected and subjected to genetic diversity evaluation using 6 simple sequence repeat (SSR). Of the genetic variation parameters, expected heterozygosity ( H e ) showed a significant variation from 0.0 in the Meshkinshahr population to 0.337 in the Andimeshk population. The analysis of molecular variance (AMOVA) revealed considerable partitioning of genetic diversity among populations (58%), while intrapopulation diversity accounted for 42%. Moderate to high population genetic differentiation was observed among particular populations, ranging from 0.060 between Marivan and Paveh to 0.562 between Meshkinshahr and Kohdasht populations. A weak total gene flow (0.115) was accompanied by a relatively high PhiPT value (0.617). Structure analysis, principal coordinate analysis, and neighbour-joining analysis, all divided the 13 populations into two genetic groups with significant genetic homogeneity. This discovery of two molecularly distinctive groups of M. minima in Iran, with a limited hybrid zone between them, is remarkable and suggests early divergent speciation.