Details

Autor(en) / Beteiligte

• Uushona, Nashipolo E.
• Dziwornu, Godwin A.
• Mkwanazi, Nonkululeko
• Kaschula, Catherine H.
• Sunassee, Suthananda N.
• de Villiers, André

Titel

New dihydroxycucurbitacin D's from the Namib desert endemic plant Acanthosicyos horridus (!nara)

Ist Teil von

• Fitoterapia, 2021-11, Vol.155, p.105041, Article 105041

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2021

Quelle

MEDLINE

Beschreibungen/Notizen

• Acanthosicyos horridus Welw. ex Hook.f. (!nara) is a leafless, thorny, melon-producing plant endemic to the hyper-arid Namib Desert. The methanol crude extract prepared from the ripe fruits of !nara afforded the known dihydroxycucurbitacin 7β-hydroxy-23,24-dihydrocucurbitacin D (1), along with four new congeners 7β,15β-dihydroxy-23,24-dihydrocucurbitacin D (2), 25-O-β-glucopyranosyl-7β-hydroxy-23,24-dihydrocucurbitacin D (3), 25-O-β-glucopyranosyl-7β-hydroxy-23,24-dihydroisocucurbitacin D (4) and 25-O-β-glucopyranosyl-7β-hydroxy-23,24-dihydro-3-epi-isocucurbitacin D (5). These compounds were isolated through a combination of preparative normal phase thin-layer chromatography (TLC) and semi-preparative reversed phase high performance liquid chromatography (HPLC). Their structures were established by comprehensive analysis of HR-ESI-MS data, 1D and 2D NMR spectroscopic data and by comparison with literature values of similar cucurbitacins. The five isolated compounds exhibited poor cytotoxic activity against the MDA-MB-231 breast cancer cell line. To the best of our knowledge, this is the first report of glycosylated cucurbitacins in Acanthosicyos horridus. [Display omitted] •Four new dihydrocucurbitacin D congeners isolated from Acanthosicyos horridus fruit.•Structures established by HR-ESI-MS data, 1D and 2D NMR spectroscopic data.•First report of glycosylated cucurbitacins in Acanthosicyos horridus (!nara).•Isolated compounds show poor cytotoxic activity to MDA-MB-231 cancer cell line.•A. horridus a source of cucurbitacin natural products of ethnobotanical significance.