Details

Autor(en) / Beteiligte

• Shesham, Vaishnavi
• Kelly, Abigail L.
• Burke, William
• Crouch, Audra
• Drake, Carrie A.
• Varaljay, Vanessa A.
• Crookes‐Goodson, Wendy J.
• Barlow, Daniel E.
• Masthay, Mark B.
• Biffinger, Justin C.

Titel

Comparison of two diphenyl polyenes as acid‐sensitive additives during the biodegradation of a thermoset polyester polyurethane coating

Ist Teil von

• Journal of applied microbiology, 2022-01, Vol.132 (1), p.351-364

Ort / Verlag

England: Oxford University Press

Erscheinungsjahr

2022

Link zum Volltext

Quelle

Wiley Blackwell Single Titles

Beschreibungen/Notizen

• Aims Biochemical hydrolysis and chemical catalysis are involved in the successful biodegradation of polymers. In order to evaluate the potential separation between biochemical and chemical catalysis during the biodegradation process, we report the use of two diphenylpolyenes (DPPs), all trans‐1,4‐diphenylbutadiene (DPB) and all trans‐1,6‐diphenylhexatriene (DPH), as potential acid‐sensitive indicators in polymers. Methods and Results 1,4‐Diphenylbutadiene and DPH (0.1% w/w) were melt‐cast successfully with poly(ethylene succinate) hexamethylene (PES‐HM) polyurethane (thermoset polyester polyurethane) coatings above 80℃. When these two DPP/PES‐HM coatings were exposed to a concentrated supernatant with significant esterase activity resulting from the growth of a recently isolated and identified strain of Tremellomycetes yeast (Naganishia albida 5307AI), the DPB coatings exhibited a measurable and reproducible localized decrease in the blue fluorescence emission in regions below where hydrolytic biodegradation was initiated in contrast with DPH blended coatings. The fluorescence changes observed in the biodegraded DPB coating were similar to exposing them to concentrated acids and not bases. Conclusions Our experiments resulted in (1) a method to blend DPP additives into thermoset coatings, (2) the first report of the biodegradation of polyester polyurethane coating by N. albida, and (3) demonstration that hydrolytic supernatants from this strain generate acidic region within degrading polyester coatings using DPB as the indicator. Significance and Impact of the Study Our experiments confirm that N. albida is an active polyester degrader and that DPB is a promising acid sensitive polymer coating additive. Acid detection in biodegrading polymers with diphenylpolyenes.