Details

Autor(en) / Beteiligte

• Rodrigues, Rafael C.
• Berenguer-Murcia, Ángel
• Carballares, Diego
• Morellon-Sterling, Roberto
• Fernandez-Lafuente, Roberto

Titel

Stabilization of enzymes via immobilization: Multipoint covalent attachment and other stabilization strategies

Ist Teil von

• Biotechnology advances, 2021-11, Vol.52, p.107821, Article 107821

Ort / Verlag

England: Elsevier Inc

Erscheinungsjahr

2021

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• The use of enzymes in industrial processes requires the improvement of their features in many instances. Enzyme immobilization, a requirement to facilitate the recovery and reuse of these water-soluble catalysts, is one of the tools that researchers may utilize to improve many of their properties. This review is focused on how enzyme immobilization may improve enzyme stability. Starting from the stabilization effects that an enzyme may experience by the mere fact of being inside a solid particle, we detail other possibilities to stabilize enzymes: generation of favorable enzyme environments, prevention of enzyme subunit dissociation in multimeric enzymes, generation of more stable enzyme conformations, or enzyme rigidification via multipoint covalent attachment. In this last point, we will discuss the features of an “ideal” immobilization protocol to maximize the intensity of the enzyme-support interactions. The most interesting active groups in the support (glutaraldehyde, epoxide, glyoxyl and vinyl sulfone) will be also presented, discussing their main properties and uses. Some instances in which the number of enzyme-support bonds is not directly related to a higher stabilization will be also presented. Finally, the possibility of coupling site-directed mutagenesis or chemical modification to get a more intense multipoint covalent immobilization will be discussed. •Enzyme immobilization in a porous structure may protect the enzyme from some inactivating causes•Enzyme immobilization may freeze some stable enzyme conformation•Multi-subunit enzyme immobilization may prevent enzyme subunit dissociation•Enzyme immobilization may enhance enzyme stability by generating special environments•Enzyme multipoint covalent attachment should increase enzyme rigidity