Details

Autor(en) / Beteiligte

• Mao, Ping
• Cao, Lei
• Li, Zedong
• You, Minli
• Gao, Bin
• Xie, Xianghong
• Xue, Zhenrui
• Peng, Ping
• Yao, Chunyan
• Xu, Feng

Titel

A digitalized isothermal nucleic acid testing platform based on a pump-free open droplet array microfluidic chip

Ist Teil von

• Analyst (London), 2021-11, Vol.146 (22), p.696-6969

Ort / Verlag

London: Royal Society of Chemistry

Erscheinungsjahr

2021

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Digital PCR has shown great potential for quantitative nucleic acid testing (NAT), but most existing platforms are dependent on large auxiliary equipment ( e.g. , vacuum pump, amplification instrument, fluorescence microscope) to achieve target dispersion, amplification, signal capture and result analysis. Such complex, expensive and bulky NAT platforms have limited their applications in resource-limited areas, especially for point-of-care testing (POCT). In this work, we designed a digital isothermal NAT platform based on a pump-free open droplet array microfluidic chip. A pump-free microfluidic chip was developed based on an open microdroplet array in the form of thousands of independent microdroplets for spontaneous sample dispersion, without the need for external power. Combined with a handheld fluorescent signal reader based on a smartphone, this digital NAT platform can accurately quantify as low as 1 copy per μL of λDNA. Therefore, our integrated NAT platform, as a potable, robust and low-cost tool for highly accurate NA quantitative analysis, holds great potential for POCT applications. A surface patterned chip for pump-free sample digitalization and open droplet array formation used for digital isothermal nucleic acid testing.