Details

Autor(en) / Beteiligte

• Sudhakar, M.P.
• Ravel, Merlyn
• Perumal, K.

Titel

Pretreatment and process optimization of bioethanol production from spent biomass of Ganoderma lucidum using Saccharomyces cerevisiae

Ist Teil von

• Fuel (Guildford), 2021-12, Vol.306, p.121680, Article 121680

Ort / Verlag

Kidlington: Elsevier Ltd

Erscheinungsjahr

2021

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• [Display omitted] •Spent mushroom substrate (SMS) of Ganoderma lucidum was used.•SMS rich in beta glucan suitable for bioethanol production.•Optimized sulphuric acid concentration and SMS percentage for acid hydrolysis.•Fermented using S. cerevisiae for ethanol production.•Final ethanol yield obtained from SMS was 0.2 % v/v. In this study, spent mushroom substrate (SMS) obtained from Ganoderma lucidum used as a potential biomass for bioethanol production was investigated. Sulphuric acid pretreatment of SMS was evaluated to optimize the sugar extraction through hydrolysis. The proximate composition of SMS was analysed. Total sugar and reducing sugar yield from SMS were calculated as 33.58 ± 0.17 mg/g and 8.28 ± 0.35 mg/g DW, respectively. Total organic carbon was recorded as 17.74 % DW. SMS pretreated with 1 % sulphuric acid yielded maximum total sugar of 807.53 ± 0.04 mg/g DW. Substrate and acid ratio was optimized for pretreatment and found that a ratio of 4:50 yielded maximum total and reducing sugar of 102.80 ± 0.73 mg/g and 12.68 ± 0.86 mg/g DW, respectively. Sugars obtained from SMS were fermented using baker’s yeast and incubated at 30 °C for 5 days. The final yield of 1.57 g L-1 of ethanol was produced on day 4 when compared with the theoretical ethanol yield of 0.91 g L-1 obtained. The present study concludes that SMS from Ganoderma lucidum considered being an alternate choice for bioethanol production to meet the biofuel demand in the future and utilization of mushroom wastes to generate energy with the concept of zero-waste discharge was emphasized.