Details

Autor(en) / Beteiligte

• Wang, Mengjun
• Zhou, Xiaobin
• Wang, Xiyang
• Wang, Mengke
• Su, Xingguang

Titel

One-step fabrication of wavelength-tunable luminescence of gold-silver bimetallic nanoclusters: Robust performance for α-glucosidase assay

Ist Teil von

• Sensors and actuators. B, Chemical, 2021-10, Vol.345, p.130407, Article 130407

Ort / Verlag

Lausanne: Elsevier B.V

Erscheinungsjahr

2021

Link zum Volltext

Quelle

Elsevier ScienceDirect Journals Complete

Beschreibungen/Notizen

• •Wavelength-tunable Au/Ag NCs were achieved via regulating the ligand content.•Both Au/Ag (g) NCs and Au/Ag (r) NCs showed superior stability and repeatability.•The Au/Ag (r) NCs/PDA nanoprobe was constructed for α-glucosidase assay.•A low detection limit of 0.007 U mL−1 was obtained for α-glucosidase assay. Herein, wavelength-tunable gold/silver bimetallic nanoclusters (Au/Ag NCs) were firstly prepared by using 5-methyl-2-thiouracil (5-MTU) as the reducing and capping agent. Notably, we found that regulating the concentration of 5-MTU could change the emission peak position of Au/Ag NCs. Green-emitting nanoclusters (Au/Ag (g) NCs) were achieved at a relatively low concentration of 5-MTU, whereas continuously increasing the 5-MTU concentration was beneficial to the formation of nanoclusters with intense red-emission (Au/Ag (r) NCs). Owing to the excellent water-solubility and superior stability of the Au/Ag (r) NCs, a sensitive ratiometric fluorescent platform for quantitative analysis of α-glucosidase was developed. In this strategy, a glycine-NaOH buffer was ingeniously adopted to trigger the dopamine (DA) self-polymerize process, which could rapidly generate polydopamine (PDA) with bright fluorescence at 470 nm and efficaciously quench the fluorescence of Au/Ag (r) NCs at 600 nm through photoinduced electron-transfer (PET) effect. By measuring the emission ratio F600/F470, this “off-on-off” sensing strategy exhibited good selectivity and sensitivity toward α-glucosidase ranging from 0.01 to 0.5 U mL−1 with quite a low detection limit (LOD) of 0.007 U mL-1. Additionally, this Au/Ag (r) NCs/PDA-based fluorometric system was utilized for α-glucosidase assay in serum samples with impressively results.