Details

Autor(en) / Beteiligte

• Prinz, Eva-Marie
• Eggers, Ruth
• Lee, Hyeck-Hee
• Steinfeld, Ute
• Hempelmann, Rolf

Titel

Synthesis of drug loaded magnetic nanoparticles and their uptake into immune cells

Ist Teil von

• Journal of physics. Conference series, 2010-01, Vol.200 (12), p.122009

Ort / Verlag

Bristol: IOP Publishing

Erscheinungsjahr

2010

Link zum Volltext

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• Ferrite nanoparticles (Mn0,8Zn0,2Fe2O4) are synthesized by the co-precipitation method and characterized by X-ray diffraction, transmission electron microscopy and dynamic light scattering. The particles are functionalized with dextran which is activated via amino or carboxymethyl groups. The chemotherapeutic drug doxorubicin (DOX) is attached to these dextran derivates in different ways. One method is based on the attachment of DOX to amino dextran by its keto group; the other is a bond to the primary amino group of DOX. The characterization of drug loaded dextran derivates is performed by Raman, FT-IR-, UV/VIS-and fluorescence spectroscopy. The biofunctionalized particles are intended for use in adoptive cancer immunotherapy as a new approach, where immune cells (T lymphocytes) will be used as new autonomous highly target specific drug delivery systems. The uptake efficiency of these particles into T lymphocytes is investigated by fluorescence and convocal microscopy.