Details

Autor(en) / Beteiligte

• Wan, Wen-Ting
• Dong, Zhi-Wei
• Ren, Yan-Dong
• Yang, Jie
• Pan, Xiang-Yu
• He, Jin-Wu
• Chang, Zhou
• Liu, Wei
• Liu, Gui-Chun
• Zhao, Ruo-Ping
• Hu, Ping
• Mao, Chu-Yang
• Li, Jun
• Wang, Wen
• Li, Xue-Yan

Titel

Chromatin accessibility profiling provides insights into larval cuticle color and adult longevity in butterflies

Ist Teil von

• Dōngwùxué yánjiū, 2021-09, Vol.42 (5), p.614-619

Ort / Verlag

Kunming: Kunming Institute of Zoology, The Chinese Academy of Sciences

Erscheinungsjahr

2021

Link zum Volltext

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• The genetic and molecular basis of phenotypic evolution is a long-standing puzzle in butterfly evolutionary studies. Since Britten and Davidson (1969) proposed the evolutionary model of regulatory sequences, increasing evidence suggests that the evolution of gene regulation leads to the formation of morphological diversity (Carroll, 2000, 2005, 2008; Carroll et al., 2005; Levine & Tjian, 2003). To explore the genetic regulatory basis of morphological divergence among butterfly species during development, we investigated chromatin accessibility in three developmental stages (i.e., 4th and 5th instar larvae and pupae) (Figure 1A) in two representative species from two different families (Papilionidae: Pap/l/o xuthus; Nymphalidae: Kall/ma /nachus) using ATAC-seq and RNA-seq. TFBSs play important roles in the regulation of gene expression mediated by transcription factors (TFs). [...]we identified the representative motifs of TFBSs in accessible peaks and predicted their potential TFs in each developmental stage and instar pair using MEME software (v5.3.2) (Machanick & Bailey, 2011). In total, 71 TFs were predicted in the three developmental stages (Supplementary Figure S2; Table 1; Supplementary Tables S5, S7) and 43 TFs binding to TFBSs were predicted within the DA ATAC-seq peaks among the instar pairs in both butterfly species (Supplementary Tables S2, S6, S8).