Details

Autor(en) / Beteiligte

• Dong, Yan-Qi
• Gao, Yu-Hong
• Zhao, Te
• Ren, Guang-Qian
• Liu, Yan-Li
• Guan, Bin
• Jin, Rong-Xian
• Gao, Fei
• Zhang, Yan-Li
• Tan, Xiu-Fang
• Zhu, Hong-Cai
• Zhang, Yu-Hong
• Zhang, Jin-Xia
• Peng, Dong
• Yan, Yu-Xin

Titel

Influencing factors and physiochemical changes of embryogenesis through in vitro isolated microspore culture in Brassica species

Ist Teil von

• Biológia, 2021-09, Vol.76 (9), p.2629-2654

Ort / Verlag

Cham: Springer International Publishing

Erscheinungsjahr

2021

Link zum Volltext

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Isolated microspore culture (IMC) is a powerful single cell system with many advantages compared with other culturing methods for Brassica species. For nearly 40 years, numerous studies have explored the influencing factors on the isolated microspore process, the ability of microspore-derived embryos (MDEs) to germinate and develop into plantlets, and the process of plant regeneration in Brassica species. In this review, the influencing factors, changes in gene expression, and the physiological and biochemical changes during the procedure are discussed at length. Ultimately, this review presents a standard protocol to improve the efficiency of IMC in Brassica haploid and double haploid (DH) production technologies.