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Development of IMBs–qPCR detection method for Yersinia enterocolitica based on the foxA gene
Ist Teil von
Archives of microbiology, 2021-09, Vol.203 (7), p.4653-4662
Ort / Verlag
Berlin/Heidelberg: Springer Berlin Heidelberg
Erscheinungsjahr
2021
Quelle
SpringerLink
Beschreibungen/Notizen
Yersinia enterocolitica
is an important zoonotic pathogen, which seriously endangers food-safety risk. In this study, the recombinant outer membrane protein OmpF and its antibody were prepared and coupled with immunomagnetic beads (IMBs) to capture
Y. enterocolitica
in food samples, combining the quantitative PCR detection with primers of virulence factor gene
fox
A for
Yersinia enterocolitica
contamination
.
The results showed that the capture efficiency of approximately 80% using anti-OmpF antibody-immunomagnetic beads and linearly dependent capture under 10
1
–10
5
CFU/mL
Y. enterocolitica
compared with less than 10% capture of other bacteria. The detection limit of 64 CFU/mL was obtained based on
fox
A gene PCR detection combined with capture of the anti-OmpF antibody-immunomagnetic beads to detect
Yersinia enterocolitica
in artificially contaminated milk and pork samples. Compared to the culture method, the developed IMBs–qPCR method has higher consistency, was less time consuming, which taken together provides an effective alternative method for rapid detection of
Y. enterocolitica
in food.