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The impact of human activities around the river can lead tothe degradation of river water quality and affect the fish blood condition. The aim of this research was toobserve the haematology profile of silver barb(Barbonymus gonionotus) caught in the Jagir River based on the water quality in each sampling station.The research was conducted by employinga survey method in Jagir River Surabaya, East Java, Indonesia. The samples were taken from three stations (10 fish each),station 1 (upstream side); station 2 residential area) and station 3 (around to paddy fields). The water quality parameters were temperature, pH, dissolved oxygen (DO), chemical oxygen demand (COD),biological oxygen demand (BOD), total suspended solids (TSS), and mercury level. Meanwhile, the haematology profile was conducted from erythrocyte, leukocytes, haemoglobin concentration, haematocrit, and micronuclei assay. The highest of erythrocyte (1,384,000 ± 145,235cells/mm3), haemoglobin (7.14 ± 0.6 g%), and haematocrit (29.5 ± 6.2%) was on the fish were captured at station 1 and the highest of leukocyte (643,288 ± 57,818cells/mm3), and micronuclei (12± 2.0 cell/1000) was on the fish at station 2, then the station 3 showed improvement the haematology profile compared to station 2.The water quality affected fish health based on their haematology profile.It is necessary to supervise and enforce the law in order to comply with the river utilization rules.