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International journal of gynecological cancer, 2014-02, Vol.24 (2), p.201-209
Ort / Verlag
Oxford: BMJ Publishing Group LTD
Erscheinungsjahr
2014
Link zum Volltext
Beschreibungen/Notizen
Objective
This study aimed to investigate the status of DNA methylation of 6 genes,
LMX1A
,
NKX6-1
,
PAX1
,
PTPRR
,
SOX1
, and
ZNF582
, previously found from squamous cell carcinomas in adenocarcinomas (ACs) of the uterine cervix.
Methods
We assessed the methylation status of these genes in 40 ACs, cervical scrapings from 23 ACs, and 67 normal control cervices by real-time quantitative methylation-specific polymerase chain reaction. The results were validated by bisulfite pyrosequencing.
Results
The methylation levels of all the 6 genes in the ACs were significantly higher than those in normal cervical tissues, especially for
PAX1
,
PTPRR
,
SOX1
, and
ZNF582
. The odds ratios and 95% confidence intervals (CIs) of high methylation levels in
PAX1
,
PTPRR
,
SOX1
, and
ZNF582
for the risk of developing an AC were 15.7 (95% CI, 7.0–40.6), 16.9 (95% CI, 7.6–43.0), 32.1 (95% CI, 12.1–124.3), and 25.4 (95% CI, 10.4–78.3), respectively (all
P
< 0.001). The methylation indices of
PAX1
,
PTPRR
,
SOX1
, and
ZNF582
recovered from scrapings of ACs were significantly higher than in normal controls. The odds ratios of these indices for the risk of developing an AC in
PAX1
,
PTPRR
,
SOX1
, and
ZNF582
were 6.2 (95% CI, 2.6–15.4), 12.1(95% CI, 3.8–46.4), 6.2 (95% CI, 2.6–15.8), and 20.6 (95% CI, 6.9–77.5), respectively (all
P
< 0.001).
Conclusions
Cervical ACs carry aberrantly high methylation rates of
PAX1
,
PTPRR
,
SOX1
, and
ZNF582
—commonly methylated in squamous cell carcinomas—which might help for AC screening.