Details

Autor(en) / Beteiligte

• Bagrov, D V
• Senkovenko, A M
• Nikishin, I I
• Skryabin, G O
• Kopnin, P B
• Tchevkina, E M

Titel

Application of AFM, TEM, and NTA for characterization of exosomes produced by placenta-derived mesenchymal cells

Ist Teil von

• Journal of physics. Conference series, 2021-06, Vol.1942 (1), p.12013

Ort / Verlag

Bristol: IOP Publishing

Erscheinungsjahr

2021

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• Abstract Exosomes and microvesicles usually require characterization at the single-particle level. The most commonly used methods for this purpose are transmission electron microscopy (TEM) and nanoparticle tracking analysis (NTA). However, atomic force microscopy (AFM) is also a promising method for the characterization of exosomes, and its opportunities seem underestimated. Here we describe the characterization of exosomes isolated from the conditioned media of human placenta cells using AFM, TEM, and NTA; we focus on the technical aspects of AFM imaging and data processing.