Details

Autor(en) / Beteiligte

• Hartmann, Stefanie A
• Martin Schaefer, H
• Segelbacher, Gernot

Titel

Development of 12 microsatellite loci for the endangered Pale-headed Brushfinch (Atlapetes pallidiceps) and their cross-amplification in two co-occurring Brushfinches

Ist Teil von

• Journal of ornithology, 2014, Vol.155 (3), p.835-839

Ort / Verlag

Berlin/Heidelberg: Springer-Verlag

Erscheinungsjahr

2014

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Using 454 pyro-sequencing, we established a microsatellite dataset of 12 polymorphic markers for the endangered Pale-headed Brushfinch (Atlapetes pallidiceps). Within 24 individuals, mean number of alleles ranged from 2 to 13, observed heterozygosity from 0.17 to 1.0, expected heterozygosity from 0.16 to 0.91, and polymorphic information content from 0.15 to 0.88. Additionally, we tested our marker set for cross-amplification in two related brushfinch species. Whereas all 12 markers amplified and were polymorphic in A. latinuchus, only 5 of the 11 markers amplified variable loci in Buarremon torquatus. This panel of microsatellites will allow a comparison between the genetic diversity of the threatened Atlapetes pallidiceps and two co-occurring non-endangered brushfinch species.