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Effect of cervical cancer-derived exosomal hsa_circ_0087432 on the proliferation and migration of HUVECs
Ist Teil von
Jie fang jun yi xue za zhi, 2021-04, Vol.46 (4), p.327
Ort / Verlag
Beijing: People's Military Medical Press
Erscheinungsjahr
2021
Link zum Volltext
Quelle
EZB Free E-Journals
Beschreibungen/Notizen
Objective To analyze the expression of circRNA0087432 (hsa_circ_0087432) in serum exosomes of patients with cervical cancer, and explore its possible relationship with cervical cancer metastasis. Methods The cervical cancer tissue and serum of 30 patients, who were diagnosed as cervical squamous cell carcinoma from June 2019 to June 2020 in Guangzhou Panyu District Central Hospital and untreated with radiotherapy and chemotherapy before surgery, were collected as the experimental group. Normal cervical tissue and serum of 30 women without cervical cancer were used as control group. Real-time quantitative polymerase chain reaction (RT-qPCR) was used to detect the expression level of hsa_circ_0087432 in cervical tissues and serum exosomes of patients with cervical cancer and healthy people; Siha cells were transfected with plasmid to overexpress hsa_circ_0087432. According to different treatments, Siha cells were divided into three groups: control group (untransfected plasmid), vector group (transfected with pL