Details

Autor(en) / Beteiligte

• Ding, Hao
• Zhou, Ping
• Fu, Wenxuan
• Ding, Lurong
• Guo, Weiliang
• Su, Bin

Titel

Spatially Selective Imaging of Cell–Matrix and Cell–Cell Junctions by Electrochemiluminescence

Ist Teil von

• Angewandte Chemie, 2021-05, Vol.133 (21), p.11875-11879

Ort / Verlag

Weinheim: Wiley Subscription Services, Inc

Erscheinungsjahr

2021

Quelle

Wiley Online Library Journals Frontfile Complete

Beschreibungen/Notizen

• Cell junctions are protein structures located at specific cell membrane domains that determine key processes in multicellular development. Here we report spatially selective imaging of cell junctions by electrochemiluminescence (ECL) microscopy. By regulating the concentrations of luminophore and/or co‐reactant, the thickness of ECL layer can be controlled to match with the spatial location of different cell junctions. At a low concentration of luminophore, ECL generation is confined to the electrode surface, thus revealing only cell–matrix adhesions at the bottom of cells. While at a high concentration of luminophore, the ECL layer can be remarkably extended by decreasing the co‐reactant concentration, thus allowing the sequential imaging of cell–matrix and cell–cell junctions at the bottom and near the apical surface of cells, respectively. This strategy not only provides new insights into the ECL mechanisms but also promises wide applications of ECL microscopy in bioimaging. The thickness of electrochemiluminescence (ECL) layer can be modulated by rationally regulating the concentrations of luminophore and/or co‐reactant to match with the spatial location of different cell junctions, thus allowing the spatially selective ECL imaging of cell–matrix adhesions at the bottom and cell–cell junctions near the apical surface of cells.