Details

Autor(en) / Beteiligte

• Huo, Fu-Chun
• Zhu, Zhi-Man
• Zhu, Wen-Tao
• Du, Qiu-Ying
• Liang, Jia
• Mou, Jie

Titel

METTL3-mediated m6A methylation of SPHK2 promotes gastric cancer progression by targeting KLF2

Ist Teil von

• Oncogene, 2021-04, Vol.40 (16), p.2968-2981

Ort / Verlag

London: Nature Publishing Group UK

Erscheinungsjahr

2021

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• N6-methyladenosine (m 6 A) RNA methylation is profoundly involved in epigenetic regulation, especially for carcinogenesis and tumor progression. Mounting evidence suggests that methyltransferase METTL3 regulates malignant behaviors of gastric cancer (GC). However, the clinical significance and biological implication of SPHK2 and its related m 6 A modification in GC remain unclear. In this study, quantitative real-time PCR (qRT-PCR), western blot and immunohistochemistry were utilized to detect the expression profiles and prognostic significance of SPHK2 in GC. Here, we showed that increased SPHK2 was signified a poor prognosis of GC patients. Phosphorylation and ubiquitination assays were used to investigate the possible mechanisms of SPHK2-mediated KLF2 expression. SPHK2 can promote the phosphorylation of KLF2, which triggers the ubiquitination and degradation of KLF2 protein in GC. Methylated RNA immunoprecipitation (MeRIP) was performed to uncover the m 6 A modification of SPHK2 mRNA. METTL3 promotes translation of SPHK2 mRNA via an m 6 A-YTHDF1-dependent manner. Functionally, SPHK2 facilitates GC cell proliferation, migration and invasion by inhibiting KLF2 expression. SPHK2/KLF2 regulates the cell proliferation, migration, and invasion induced by METTL3 in GC. Overall, our findings reveal that METTL3-mediated m 6 A modification of SPHK2 contributes to GC progression, which extends the understanding of the importance m 6 A methylation in GC and represents a potential target for GC therapy.