Details

Autor(en) / Beteiligte

• Zhou, Bin
• Thao, Tran Thi Nhu
• Hoffmann, Donata
• Taddeo, Adriano
• Ebert, Nadine
• Labroussaa, Fabien
• Pohlmann, Anne
• King, Jacqueline
• Steiner, Silvio
• Kelly, Jenna N
• Portmann, Jasmine
• Halwe, Nico Joel
• Ulrich, Lorenz
• Trüeb, Bettina Salome
• Fan, Xiaoyu
• Hoffmann, Bernd
• Wang, Li
• Thomann, Lisa
• Lin, Xudong
• Stalder, Hanspeter
• Pozzi, Berta
• de Brot, Simone
• Jiang, Nannan
• Cui, Dan
• Hossain, Jaber
• Wilson, Malania M
• Keller, Matthew W
• Stark, Thomas J
• Barnes, John R
• Dijkman, Ronald
• Jores, Joerg
• Benarafa, Charaf
• Wentworth, David E
• Thiel, Volker
• Beer, Martin

Titel

SARS-CoV-2 spike D614G change enhances replication and transmission

Ist Teil von

• Nature (London), 2021-04, Vol.592 (7852), p.122-3

Ort / Verlag

England: Nature Publishing Group

Erscheinungsjahr

2021

Quelle

MEDLINE

Beschreibungen/Notizen

• During the evolution of SARS-CoV-2 in humans, a D614G substitution in the spike glycoprotein (S) has emerged; virus containing this substitution has become the predominant circulating variant in the COVID-19 pandemic . However, whether the increasing prevalence of this variant reflects a fitness advantage that improves replication and/or transmission in humans or is merely due to founder effects remains unknown. Here we use isogenic SARS-CoV-2 variants to demonstrate that the variant that contains S(D614G) has enhanced binding to the human cell-surface receptor angiotensin-converting enzyme 2 (ACE2), increased replication in primary human bronchial and nasal airway epithelial cultures as well as in a human ACE2 knock-in mouse model, and markedly increased replication and transmissibility in hamster and ferret models of SARS-CoV-2 infection. Our data show that the D614G substitution in S results in subtle increases in binding and replication in vitro, and provides a real competitive advantage in vivo-particularly during the transmission bottleneck. Our data therefore provide an explanation for the global predominance of the variant that contains S(D614G) among the SARS-CoV-2 viruses that are currently circulating.