Details

Autor(en) / Beteiligte

• Melo, Sebastião J
• Sousa, João Paulo B
• Sá, Maíra G
• Morais, Laís S
• Magalhães, Natália MG
• Gouveia, Fernando N
• Albernaz, Lorena C
• Espindola, Laila S

Titel

Machaerium acutifolium compounds with larvicidal activity against Aedes aegypti

Ist Teil von

• Pest management science, 2021-03, Vol.77 (3), p.1444-1451

Ort / Verlag

Chichester, UK: John Wiley & Sons, Ltd

Erscheinungsjahr

2021

Link zum Volltext

Quelle

Wiley Online Library All Journals

Beschreibungen/Notizen

• BACKGROUND Plant extracts and isolated compounds are known for their insecticidal activity. The Aedes aegypti mosquito has a significant medical impact as it transmits a number of arboviruses and is able to develop resistance to the commercially available insecticides. This study investigates larvicidal compounds isolated from Machaerium acutifolium, designated by the Brazilian Forest Service as a sustainable species. RESULTS A M. acutifolium trunk ethyl acetate extract was fractionated using chromatographic methods with full structural elucidation by mass spectrometry (MS), nuclear magnetic resonance and specific rotation analyses revealing: one new 3‐arylcoumarin derivative 1; two flavonoids 2 and 3; a trans‐stilbene 4, and an unprecedented natural indene 5. The larvicidal activity against Ae. aegypti after 24 h exposure was: crude extract (median lethal dose, LC50 205 mg L–1), fraction C (LC50 27 mg L–1) and 5 (LC50 24 mg L–1). CONCLUSION A M. acutifolium extract showed larvicidal activity, which increased with prolonged exposure, demonstrating LC50 75 mg L–1 after 72 h. Although the flavonoids 2 and 3 and trans‐stilbene 4 were deemed inactive according to the adopted mortality limit, additional tests revealed their ability to cause 65% Ae. aegypti larvae mortality, suggesting they could contribute to the larvicidal activity. Compound 5, identified by liquid chromatography‐MS, was over eight‐fold more toxic to larvae than the crude extract after 24 h. Therefore, 5 constitutes a structural model for new prototypes to control Ae. aegypti. These data reinforce the potential of natural products as a source of commercial alternatives for vector control strategies, respecting both sustainability and eco‐friendly principles. © 2020 Society of Chemical Industry A Machaerium acutifolium trunk extract and a previously unreported natural indene demonstrated increasing larvicidal activity over prolonged exposure resulting in an eco‐friendly sustainable candidate to control Aedes aegypti.