Details

Autor(en) / Beteiligte

• Ju, Peng
• Zhang, Yu
• Ding, Jinfeng
• Zheng, Yifan
• Wang, Shuai
• Jiang, Fenghua
• Sun, Chengjun

Titel

New insights into the toxic interactions of polyvinyl chloride microplastics with bovine serum albumin

Ist Teil von

• Environmental science and pollution research international, 2021-02, Vol.28 (5), p.5520-5531

Ort / Verlag

Berlin/Heidelberg: Springer Berlin Heidelberg

Erscheinungsjahr

2021

Quelle

SpringerLink

Beschreibungen/Notizen

• The binding interaction between emerging pollutant polyvinyl chloride microplastics (PVC MPs) and bovine serum albumin (BSA) was studied by fluorescence spectroscopy, resonance scattering spectroscopy (RLS), UV-visible (UV-vis) absorption spectroscopy, circular dichroism (CD), and Fourier transform infrared (FT-IR) spectroscopy under simulative physiological conditions. Fluorescence results revealed that the fluorescence quenching of BSA induced by PVC MPs was originated from the formation of BSA-PVC complex in static quenching mode. According to Stern-Volmer equation, the binding constants ( K a ) between PVC MPs and BSA at different temperatures were obtained, and the number of binding sites was 1.62. The thermodynamic parameters, enthalpy change (Δ H ), entropy change (Δ S ), and free energy change (Δ G ) were calculated to be − 41.77 kJ mol -1 , 43.17 J mol -1 K -1 , and − 54.63 kJ mol -1 via Van’t Hoff equation, indicating electrostatic interaction played a key role in the formation of BSA-PVC complex spontaneously. In addition, the alterations of microenvironment and secondary structure in BSA induced by PVC MPs were further confirmed by synchronous fluorescence spectra, UV-vis, FT-IR, and CD. This work not only provides further information for better understanding the binding interaction of PVC MPs with BSA, but also elucidates the potential biological toxicity of MPs at a molecular level.