Details

Autor(en) / Beteiligte

• Poma, Noemi
• Vivaldi, Federico
• Bonini, Andrea
• Salvo, Pietro
• Kirchhain, Arno
• Melai, Bernardo
• Bottai, Daria
• Tavanti, Arianna
• Di Francesco, Fabio

Titel

A graphenic and potentiometric sensor for monitoring the growth of bacterial biofilms

Ist Teil von

• Sensors and actuators. B, Chemical, 2020-11, Vol.323, p.128662, Article 128662

Ort / Verlag

Lausanne: Elsevier B.V

Erscheinungsjahr

2020

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• [Display omitted] •A graphenic and potentiometric sensor monitors the bacterial biofilm formation.•The open circuit potential responded to bacterial growth in real-time.•Negative correlation found between OCP and number of bacteria.•Monitoring effectively performed both in liquid and semisolid culture media. Biofilm development on surfaces represents one of the most challenging issues in the medical field. The irreversibility of the biofilm developmental process, and the peculiar characteristics of biofilms (including the low permeability to antibacterial compounds), make biofilm associated infections difficult to treat and prone to relapse. Thanks to the short response time, low-cost, easy fabrication and potential for miniaturization, electrochemical sensors are expected to play an important role in monitoring biofilm formation. Here, we describe the use of a potentiometric sensor based on functionalized reduced graphene oxide for monitoring the formation and growth of biofilms from three bacterial species (Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus) in liquid and semisolid culture media. The Open Circuit Potential (OCP) between an Ag/AgCl pseudo reference electrode and a working electrode, both screen-printed on a flexible polyethylene terephthalate film, was measured during the growth of bacteria. The OCP decreased over time as bacteria grew on the sensor surface exhibiting a negative correlation.