Details

Autor(en) / Beteiligte

• Bhardwaj, Dinesh Kumar
• Taneja, Neetu Kumra
• DP, Shivaprasad
• Chakotiya, Ankita
• Patel, Praveen
• Taneja, Pankaj
• Sachdev, Divya
• Gupta, Sarita
• Sanal, Madhusudana Girija

Titel

Phenotypic and genotypic characterization of biofilm forming, antimicrobial resistant, pathogenic Escherichia coli isolated from Indian dairy and meat products

Ist Teil von

• International journal of food microbiology, 2021-01, Vol.336, p.108899, Article 108899

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2021

Quelle

Access via ScienceDirect (Elsevier)

Beschreibungen/Notizen

• Escherichia coli are commensal gastrointestinal microflora of humans, but few strains may cause food-borne diseases. Present study aimed to identify antimicrobial resistant (AMR), biofilm-forming E. coli from Indian dairy and meat products. A total of 32 E. coli isolates were identified and evaluated for biofilm-formation. EMC17, an E. coli isolate was established as a powerful biofilm-former that attained maximum biofilm-formation within 96 h on glass and stainless-steel surfaces. Presence and expression of virulence-associated genes (adhesins, invasins and polysaccharides) and ability to adhere and invade human liver carcinoma HepG2 cell lines implicates EMC17 to be pathotype belonging to Extra-intestinal Pathogenic E. coli (ExPEC). Antibiotic profiling of EMC17 identified it as multi-drug resistant (MDR) strain, possessing extended spectrum β-lactamases (ESBL's) and biofilm phenotype. Early production of quorum sensing molecules (AHLs) alongside EPS production facilitated early onset of biofilm formation by EMC17. Furthermore, the biofilm-forming genes of EMC17 were significantly upregulated 3–27 folds in the biofilm-state. This study showed prevalence of MDR, biofilm-forming, pathogenic E. coli in Indian dairy and meat products that potentially serve as reservoirs for transmission of antimicrobial-resistant (AMR) genes of bacteria from food to humans and pose serious food safety threat. •Out of 60 products tested 40–60% dairy and 60% meat showed presence of E. coli.•β-Lactam drug resistance was the most commonly observed phenotype.•E. coli EMC 17 was characterized as MDR, biofilm-forming pathogenic ExPEC strain.•EMC17 carries transferrable ESBL gene blaCMY2 escalating AMR spread to other bacteria.•Static EMC17 augmented biofilm gene expression, adherence, AHL & EPS production.