UNIVERSI
TÄ
TS-
BIBLIOTHEK
P
ADERBORN
Anmelden
Menü
Menü
Start
Hilfe
Blog
Weitere Dienste
Neuerwerbungslisten
Fachsystematik Bücher
Erwerbungsvorschlag
Bestellung aus dem Magazin
Fernleihe
Einstellungen
Sprache
Deutsch
Deutsch
Englisch
Farbschema
Hell
Dunkel
Automatisch
Sie befinden Sich nicht im Netzwerk der Universität Paderborn. Der Zugriff auf elektronische Ressourcen ist
gegebenenfalls
nur via VPN oder Shibboleth (DFN-AAI) möglich.
mehr Informationen...
Universitätsbibliothek
Katalog
Suche
Details
Zur Ergebnisliste
Ergebnis 18 von 95
Datensatz exportieren als...
BibTeX
Identification and characterization of striking multiple‐insecticide resistance in a Tetranychus urticae field population from Greece
Pest management science, 2021-02, Vol.77 (2), p.666-676
Papapostolou, Kyriaki Maria
Riga, Maria
Charamis, Jason
Skoufa, Evangelia
Souchlas, Vassilis
Ilias, Aris
Dermauw, Wannes
Ioannidis, Panagiotis
Van Leeuwen, Thomas
Vontas, John
2021
Volltextzugriff (PDF)
Details
Autor(en) / Beteiligte
Papapostolou, Kyriaki Maria
Riga, Maria
Charamis, Jason
Skoufa, Evangelia
Souchlas, Vassilis
Ilias, Aris
Dermauw, Wannes
Ioannidis, Panagiotis
Van Leeuwen, Thomas
Vontas, John
Titel
Identification and characterization of striking multiple‐insecticide resistance in a Tetranychus urticae field population from Greece
Ist Teil von
Pest management science, 2021-02, Vol.77 (2), p.666-676
Ort / Verlag
Chichester, UK: John Wiley & Sons, Ltd
Erscheinungsjahr
2021
Quelle
MEDLINE
Beschreibungen/Notizen
BACKGROUND Tetranychus urticae is a notorious crop pest with a worldwide distribution that has developed resistance to a wide range of acaricides. Here, we investigated the resistance levels of a T. urticae population collected from an ornamental greenhouse in Peloponnese, Greece, and analyzed its resistance mechanisms at the molecular level. RESULTS Toxicological assays showed resistance against compounds with different modes of action, with resistance ratios of: 89‐fold for abamectin; > 1000‐fold for clofentezine; > 5000‐fold for etoxazole; 27‐fold for fenpyroximate and pyridaben; 20‐ and 36‐fold for spirodiclofen and spirotetramat, respectively; and 116‐ and > 500‐fold for cyenopyrafen and cyflumetofen, respectively. Bioassays with synergists indicated the involvement of detoxification enzymes in resistance to abamectin, but not to cyflumetofen and spirodiclofen. RNA sequencing (RNA‐seq) analysis showed significant over‐expression of several genes encoding detoxification enzymes such as cytochrome P450 monooxygenases and UDP‐glycosyltransferases, which have been previously associated with acaricide resistance. Known target‐site resistance mutations were identified in acetyl‐choline esterase, chitin synthase 1 and NDUFS7/psst, but putative novel resistance mutations were also discovered in targets such as glutamate‐gated chloride channel subunit 3. Interestingly, target‐site resistance mutations against pyrethroids or bifenazate were not identified, possibly indicating a recent reduced selection pressure in Greece, as well as a possible opportunity to rotate these chemistries. CONCLUSION We identified and characterized a striking case of multiple acaricide resistance in a field population of T. urticae. Exceptionally strong resistance phenotypes, with accumulation of multiple resistance mutations and over‐expression of P450s and other detoxification genes in the same field population are reported. A Tetranychus urticae field population from Greece shows striking resistance levels against multiple acaricides with different modes of action, due to accumulated target‐site mutations and over‐expression of detoxification genes. © 2020 Society of Chemical Industry
Sprache
Englisch
Identifikatoren
ISSN: 1526-498X
eISSN: 1526-4998
DOI: 10.1002/ps.6136
Titel-ID: cdi_proquest_journals_2477160708
Format
–
Schlagworte
Abamectin
,
acaricide
,
Acaricides
,
Acaricides - pharmacology
,
Animals
,
Bioassays
,
Chitin
,
Chitin synthase
,
Chloride ions
,
Choline
,
Cytochrome
,
Cytochrome P450
,
Cytochromes P450
,
Detoxification
,
Enzymes
,
Esterase
,
Fungicides
,
Gene expression
,
Gene sequencing
,
Genes
,
Greece
,
Insecticide resistance
,
Insecticide Resistance - genetics
,
Insecticides
,
Ion channels
,
multi‐resistance
,
Mutation
,
mutations
,
Pesticide resistance
,
Phenotypes
,
Population
,
Pyrethrins
,
Pyrethroids
,
Pyridaben
,
Ribonucleic acid
,
RNA
,
Synergists
,
Tetranychidae - genetics
,
Tetranychus urticae
,
transcriptomics
,
two‐spotted spider mite
Weiterführende Literatur
Empfehlungen zum selben Thema automatisch vorgeschlagen von
bX