Details

Autor(en) / Beteiligte

• Saha, Partha
• Ghoshal, Chandrika
• Ray, Soham
• Saha, Namita Das
• Srivastava, Mohita
• Kalia, Pritam
• Tomar, B. S.

Titel

Genetic analysis of downy mildew resistance and identification of molecular markers linked to resistance gene Ppa207 on chromosome 2 in cauliflower

Ist Teil von

• Euphytica, 2020-11, Vol.216 (11), Article 183

Ort / Verlag

Dordrecht: Springer Netherlands

Erscheinungsjahr

2020

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Downy mildew is one of the most damaging fungal diseases of cauliflower. The present study was undertaken to identify molecular markers linked to the downy mildew resistance gene using 84 recombinant inbred lines (RILs) from a cross of Pusa Sharad (susceptible [S] to downy mildew) and DMR-2-0-7 (resistant [R] to downy mildew). The resistant and susceptible lines in the 84 RILs segregated in 1:1 (R:S) ratio, indicating that resistance for downy mildew disease is monogenic and the gene was named as  Ppa 207 . During polymorphism survey between the parents using 105 simple sequence repeat (SSR) markers, 43 were polymorphic (27.55%). Bulked segregant analysis revealed that two of these markers that showed polymorphism, namely BoGMS0486 and BoGMS0900, could distinguish resistant and susceptible bulks and segregated in 1:1 (R:S) ratio. The recombination frequencies between BoGMS0486 and BoGMS0900, BoGMS0486 and Ppa 207 and BoGMS0900 and Ppa 207 were 4.8, 3.6 and 1.2%, respectively. The resistant gene Ppa 207 was mapped in 4.8 cM linkage interval on linkage group 2 (C02) of cauliflower, flanked by the markers BoGMS0486 and BoGMS0900 at 3.6 and 1.2 cM, respectively, from Ppa 207 . Markers BoGMS0486 and BoGMS0900 were located 2.9 and 23.2 Mb positions, respectively, on chromosome 2 and the 4.8 cM marker interval corresponds to a physical distance of 20.3 Mb. The identified SSR markers linked to the new resistance gene Ppa 207 will be very useful in marker assisted selection in cauliflower for developing varieties and/or hybrids resistant to downy mildew.