Details

Autor(en) / Beteiligte

• Zhang, Yi-Wen
• Liu, Wen-Sheng
• Chen, Jing-Shuai
• Niu, He-Lin
• Mao, Chang-Jie
• Jin, Bao-Kang

Titel

Metal-organic gel and metal-organic framework based switchable electrochemiluminescence RNA sensing platform for Zika virus

Ist Teil von

• Sensors and actuators. B, Chemical, 2020-10, Vol.321, p.128456, Article 128456

Ort / Verlag

Lausanne: Elsevier B.V

Erscheinungsjahr

2020

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• •The 3D chromophore connectivity in Zr-MOG could promoted the charge migration and recombination among the excited-state of g-C3N4 units.•The combination of Zr-MOG and g-C3N4 units could obtain highly efficient solid-state ECL.•MOFs accept the ECL of nanocomposite via efficient RET, and consume co-reactant, leading to the dual ECL quenching effect.•Signal was amplified by using endonuclease IV to circularly cleave the AP site of cDNA upon the formation of RNA/cDNA duplex. In this work, an ultrasensitive switchable electrochemiluminescence (ECL) RNA sensing platform was established based on metal-organic gel (MOG) and metal-organic framework (MOF) as electrode matrix and nanotag, respectively. The former was prepared via the in-situ loading of graphite-like carbon nitride (g-C3N4) and post-assembly of Au nanoparticles (AuNPs) within Zr-based MOG (AuNPs&g-C3N4@Zr-MOG), exhibiting highly efficient solid-state ECL. Fe-MIL-88 MOFs not only act as an ECL acceptor in ECL resonance energy transfer system but also have metal active centers to consume co-reactant, initiating double quenching effect on the ECL of AuNPs&g-C3N4@Zr-MOG. Then, DNA probe containing an apurinic/ apyrimidinic (AP) site connected the both, resulting in a turn-off signal. In the presence of the target RNA, the ECL was turned on because the AP site of DNA probe was activated to be circularly cleaved by endonuclease IV. Using Zika virus (ZIKV) RNA as a model analyte, the ECL platform showed a wide detection range from 0.3 nM to 3 μM with a detection limit as low as 0.1 nM. Besides, the sensor exhibited excellent specificity and stability, as well as acceptable real sample detection capability, illustrating the feasibility of clinical measurement in the future.