Details

Autor(en) / Beteiligte

• Mesquita, Felipe Pantoja
• Moreira-Nunes, Caroline Aquino
• da Silva, Emerson Lucena
• Lima, Luina Benevides
• Daniel, Júlio Paulino
• Zuerker, William J.
• Brayner, Mirna
• de Moraes, Maria Elisabete Amaral
• Montenegro, Raquel Carvalho

Titel

MAPK14 (p38α) inhibition effects against metastatic gastric cancer cells: A potential biomarker and pharmacological target

Ist Teil von

• Toxicology in vitro, 2020-08, Vol.66, p.104839, Article 104839

Ort / Verlag

England: Elsevier Ltd

Erscheinungsjahr

2020

Quelle

MEDLINE

Beschreibungen/Notizen

• Gastric cancer has been considering one of the worst cancer types since it is diagnosed in advanced stages, currently in the metastatic stage. Therefore, the challenge is to find out a biomarker and a pharmacology target that would help face this worldwide health issue. In this sense, the mitogen-activated protein kinase (MAPK) signaling pathway has become an important aim of the studies in several cancers. Therefore, we evaluated the role of MAPK14 (p38α) inhibitor SB-245392 in the cellular process, such as proliferation, cell death, and cell migration, and whether MAPK14 gene could be a potential biomarker in gastric cancer models. The results clearly suggest that p38α inhibition significantly impairs the cell proliferation, induces modest apoptosis and strongly inhibits cell migration of gastric cancer cell (AGP-01). Gene expression analysis showed that c-MYC level was decreased and TP53 was increased after SB-245392 treatment. Furthermore, MAPK14 was found in high levels in gastric cancer samples compared to normal samples in the TCGA database, especially in advanced stages (stage 3 and 4), which is significantly associated with low rate survival of the patients. In conclusion, the MAPK14 could be a potential biomarker for advanced gastric cancer as well as a pharmacological target, which could improve the survival rate of patients. •p38α inhibition decreased the gastric cancer cell proliferation by provoking cell cycle arrest and cell death.•Cellular migration of gastric cancer cells was impaired by p38α inhibition.•Overexpression of MAPK14 (p38α) gene analyzed in TGCA databank was directly associated with poor survival.