Details

Autor(en) / Beteiligte

• Oh, Sewon
• Oh, Youngjae
• Kim, Keumsun
• Han, Hyeondae
• Kim, Yumi
• Won, Kyungho
• Kim, Daeil

Titel

Construction of high-resolution genetic linkage map in pear pseudo-BC1 ((Pyrus pyrifolia × P. communis) × P. pyrifolia) using GBS-SNPs and SSRs

Ist Teil von

• Horticulture, environment and biotechnology, 2020-08, Vol.61 (4), p.745-753

Ort / Verlag

Singapore: Springer Singapore

Erscheinungsjahr

2020

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Genetic linkage map of pear (( Pyrus pyrifolia  ×  P. communis ) ×  P. pyrifolia ) was constructed to validate the effectiveness of the two-enzyme approach in GBS library preparation. In addition, allele inheritance was analyzed to investigate the usefulness of the pear pseudo-BC 1 in genetic analysis. A total of 905 GBS-SNPs and 69 SSRs were anchored in 17 linkage groups with total genetic distance of 1760.1 cM and average marker distance of 1.88 cM. The genetic linkage map represents 80.5% of pear genome. GBS two-enzyme approach improved marker density and genome coverage compared to the single-enzyme approach. In addition, the inheritance analysis of SSR alleles demonstrated that pseudo-BC could allow genetic dissection of donor parent specific trait segregating in the F 2 . The high-resolution genetic linkage map of pear pseudo-BC 1 will be used for candidate gene identification. Moreover, genetic analysis of donor parent specific traits segregating in the pseudo-BC 1 hybrids will promote marker development for molecular breeding in pear.