Details

Autor(en) / Beteiligte

• Zhang, Yiling
• Shang, Ruisha
• Zhang, Jiao
• Li, Junhao
• Zhu, Guanyu
• Yao, Mingshuai
• Sun, Jiancheng
• Shen, Zhongyuan

Titel

Isolation and identification of two Serratia marcescens strains from silkworm, Bombyx mori

Ist Teil von

• Antonie van Leeuwenhoek, 2020-09, Vol.113 (9), p.1313-1321

Ort / Verlag

Cham: Springer International Publishing

Erscheinungsjahr

2020

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Bacterial septicemia commonly occurs and usually cause huge losses in sericulture industry. Here, two pathogenic bacterial strains were isolated from dead silkworm and named as ZJ-1 and ZJ-2. Phenotypic and genotypic analysis results revealed that both of these two strains are closely related to Serratia marcescens ( S. marcescens ). The morphological as well as physiological and biochemical characteristics of ZJ-1 were accordant with S. marcescens mentioned in Bergey’s manual of determinative bacteriology, whereas ZJ-2 showed some discrepancies such as the utilization of malonate and starch, fermentation of maltose and sucrose, and tests of urease, etc. Surprisingly, ZJ-2 could produce red pigment at high temperature (37°) but ZJ-1 could not. Besides, by analyzing the lethal concentration 50 (LC 50 ) of ZJ-1 and ZJ-2, it was found that the virulence of ZJ-2 was lower than that of ZJ-1. These results revealed that ZJ-1 and ZJ-2 were two different strains of S. marcescens and that ZJ-2 was expected to be a safe (low-toxicity) and efficient strain for the production of red pigment. Nonetheless, further research in molecular level is needed to understand the regulation mechanism of pigment production and infection of ZJ-2.