Details

Autor(en) / Beteiligte

• Suganthi, M.
• Senthilkumar, P.
• Abirami, G.
• Thenmozhi, M.
• Jayanthi, M

Titel

Isolation and Screening of chitinase producing endophytic bacteria from Datura metal L

Ist Teil von

• Research journal of pharmacy and technology, 2020-03, Vol.13 (3), p.1243-1247

Ort / Verlag

Raipur: A&V Publications

Erscheinungsjahr

2020

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Chitin is widely distributed in nature and it is the principle structural component of outer skeleton (50% of the cuticle made up of chitin), foregut, hindgut, midgut lining of peritrophic membrane and also essential for structural integrity of many insects, nematodes[2] as well as component of the cell wall of the most fungi[3]. A total of 18 different bacterial strains from root and 17 different bacterial strains from leaves of Datura were isolated. [...]the bacterial strains were named as DR-1 (D. metal L. root) to DR-17 for the bacterial strains isolated from root samples and DL-1 (D. metal L. leaf) to DL-17 for the bacterial strains isolated from leaf samples. Chitinase production was determined by zones of hydrolysis produced after 96 h of incubation at 37°C. The result of this investigation revealed thirty-five pure bacterial isolates from the leaf and root (Table. 1 and 2) were found to produce clear zone (>10 mm) when incubated in chitin-containing media and further stained with Congo red (Fig. 3 and 4). Due to high toxicity of chemical pesticide to non target organisms such as human beings, animals and beneficial insects, the use of chemical pesticides is being replaced by environment friendly biopesticides.