Details

Autor(en) / Beteiligte

• Zhang, Yutao
• Yan, Chenxu
• Wang, Chao
• Guo, Zhiqian
• Liu, Xiaogang
• Zhu, Wei‐Hong

Titel

A Sequential Dual‐Lock Strategy for Photoactivatable Chemiluminescent Probes Enabling Bright Duplex Optical Imaging

Ist Teil von

• Angewandte Chemie International Edition, 2020-06, Vol.59 (23), p.9059-9066

Auflage

International ed. in English

Ort / Verlag

Germany: Wiley Subscription Services, Inc

Erscheinungsjahr

2020

Quelle

MEDLINE

Beschreibungen/Notizen

• Chemiluminescence (CL)‐based technologies have revolutionized in vivo monitoring of biomolecules. However, significant technical hurdles have limited the achievement of trigger‐controlled, bright, and enriched CL signal. Herein, a dual‐lock strategy uses sequence‐dependent triggers for bright optical imaging with real‐time fluorescent signal and ultra‐sensitive CL signal. These probes can obtain an analyte‐triggered accumulation of stable pre‐chemiluminophore with aggregation‐induced emission (AIE), and then the pre‐chemiluminophore exhibits a rapid photooxidation process (1,2‐dioxetane generation) by TICT‐based free‐radical addition, thereby achieving an enrichment and bright CL signal. The dual‐lock strategy expands the in vivo toolbox for highly accurate analysis and has for the first time allowed access to accurately sense and trace biomolecules with high‐resolution, dual‐mode of chemo‐fluoro‐luminescence, and three‐dimensional (3D) imaging in living animals. A dual‐lock strategy by sequence‐dependent triggers is developed for bright optical imaging with the integration of real‐time fluorescence and ultra‐sensitive chemiluminescence (CL) signals. This sequential dual‐lock strategy allows accurate sensing and tracing of biomolecules with high‐resolution, dual‐mode 3D imaging in living animals.