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Isolation and application of bacteriophages alone or in combination with nisin against planktonic and biofilm cells of Staphylococcus aureus
Ist Teil von
Applied microbiology and biotechnology, 2020-06, Vol.104 (11), p.5145-5158
Ort / Verlag
Berlin/Heidelberg: Springer Berlin Heidelberg
Erscheinungsjahr
2020
Link zum Volltext
Quelle
SpringerLink (Online service)
Beschreibungen/Notizen
Staphylococcus aureus
is a notorious foodborne pathogen since it has ability to produce variety of toxins including heat-stable enterotoxin, form biofilm, and acquire resistance to antibiotics. Biocontrol of foodborne pathogens by lytic bacteriophages garners increasing interest from both researchers and food industry. In the present study, 29 phages against
S. aureus
were successfully isolated from chicken, pork, and fish. Characterization of the isolates revealed that phage SA46-CTH2 belonging to
Podoviridae
family had a number of features suitable for food industry applications such as wide host range, short latent period, large burst size, high stress tolerance, and a genome free of virulence genes. Furthermore, phage SA46-CTH2 alone or in combination with nisin exhibited great efficacy in reducing planktonic and biofilm cells of
S. aureus
at various conditions tested. The combination of phage SA46-CTH2 and nisin was also found to be able to inhibit the regrowth of
S. aureus
at both 37 and 24 °C.
Key points
• A total of 29 S. aureus phages were successfully isolated from fish, pork, and chicken products.
• Phage SA46-CTH2 was characterized by host range, morphology, and genome sequencing.
• SA46-CTH2 significantly reduced both planktonic and biofilm cells of S. aureus.
• Combination of SA46-CTH2 and nisin inhibited the regrowth of S. aureus.