Details

Autor(en) / Beteiligte

• Krivitskaya, V. Z.
• Sorokin, E. V.
• Tsareva, T. R.
• Sergeeva, M. V.
• Kadyrova, R. A.
• Romanovskaya-Roman’ko, E. A.
• Shaldzhyan, A. A.
• Petrov, S. V.
• Petrova, E. R.
• Konovalova, N. I.
• Petrova, P. A.

Titel

Generation and Characterization of the Monoclonal Antibody Panel Specific to the NS1 Protein of the Influenza A Virus

Ist Teil von

• Applied biochemistry and microbiology, 2018-12, Vol.54 (7), p.756-765

Ort / Verlag

Moscow: Pleiades Publishing

Erscheinungsjahr

2018

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• The paper reports on the production of a panel of seven new monoclonal antibodies (MAbs) capable of specifically binding to the NS1 1–124 fragment of the influenza virus A/Puerto Rico/8/1934 (H1N1) NS1 protein in indirect enzyme-linked immunosorbent assay (ELISA), as well as to the full-size native NS1 protein in cells infected with different subtypes of the influenza A virus (A(H1N1), A(H1N1)pdm09, A(H2N2), A(H3N2), and A(H9N2)). A sensitive variant of the microcultural ELISA based on the use of the generated MAbs is proposed; it may be utilized to detect NS1 protein in infected cells and to study its antigenic variability. The results of the performed microcultural ELISA demonstrate type- and subtype-specific antigenic variability of the influenza virus NS1 protein.