Details

Autor(en) / Beteiligte

• Bednařík, Antonín
• Preisler, Jan
• Bezdeková, Dominika
• Machálková, Markéta
• Hendrych, Michal
• Navrátilová, Jarmila
• Knopfová, Lucia
• Moskovets, Eugene
• Soltwisch, Jens
• Dreisewerd, Klaus

Titel

Ozonization of Tissue Sections for MALDI MS Imaging of Carbon–Carbon Double Bond Positional Isomers of Phospholipids

Ist Teil von

• Analytical chemistry (Washington), 2020-05, Vol.92 (9), p.6245-6250

Ort / Verlag

United States: American Chemical Society

Erscheinungsjahr

2020

Quelle

MEDLINE

Beschreibungen/Notizen

• Visualizing the differential distribution of carbon–carbon double bond (CC db) positional isomers of unsaturated phospholipids (PL) in tissue sections by use of refined matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI MSI) technologies offers a high promise to deeper understand PL metabolism and isomer-specific functions in health and disease. Here we introduce an on-tissue ozonization protocol that enables a particular straightforward derivatization of unsaturated lipids in tissue sections. Collision-induced dissociation (CID) of MALDI-generated ozonide ions (with yields in the several ten percent range) produced the Criegee fragment ion pairs, which are indicative of CC db position(s). We used our technique for visualizing the differential distribution of Δ9 and Δ11 isomers of phosphatidylcholines in mouse brain and in human colon samples with the desorption laser spot size 15 μm, emphasizing the potential of the technique to expose local isomer-specific metabolism of PLs.