Details

Autor(en) / Beteiligte

• Borisova, O. V.
• Galstyan, A. G.
• Olenin, A. Yu
• Lisichkin, G. V.
• Zverev, V. V.

Titel

Differential Diagnostics of Bacteria Using the Surface-Enhanced Raman Spectra

Ist Teil von

• Microbiology (New York), 2020-03, Vol.89 (2), p.192-196

Ort / Verlag

Moscow: Pleiades Publishing

Erscheinungsjahr

2020

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• — Surface-enhanced Raman scattering of bioorganic compounds close to silver nanoparticles may be used for species identification of microbial colonies. The preparations of silver nanoparticles sol with a single microbial colony (strains Escherichia coli 376/2, Staphylococcus aureus 25923 , Pseudomonas aeruginosa ATCC 27583, and Bacillus subtilis 534) exhibited the individual Raman spectra, in which the band occurrence and intensity correlated with the species composition. The most important differences in the spectra of these four strains were associated with the bands at 649‒652, 948‒950, 1326‒1340, and 1468 cm −1 . Application of the principal component analysis within the range from 600 to 1600 cm −1 resulted in the sum of the factor load of two major components equal to 83.53% of the total differences between the spectra and demonstrated the possibility of differential diagnostics of four microbial species.