Sie befinden Sich nicht im Netzwerk der Universität Paderborn. Der Zugriff auf elektronische Ressourcen ist gegebenenfalls nur via VPN oder Shibboleth (DFN-AAI) möglich. mehr Informationen...
Relationship of Arachidonic Acid Concentration to Cyclooxygenase-Dependent Human Platelet Aggregation
Ist Teil von
Journal of clinical pharmacology, 2003-09, Vol.43 (9), p.983-989
Ort / Verlag
Oxford, UK: Blackwell Publishing Ltd
Erscheinungsjahr
2003
Link zum Volltext
Quelle
MEDLINE
Beschreibungen/Notizen
Inhibition of ex vivo arachidonic acid (AA)–induced aggregation is a biomarker for the isotype selectivity of cyclooxygenase (COX) inhibitors since platelets express COX‐1 but not COX‐2. At low concentrations, there is broad inter‐ and intrasubject variability in AA‐induced aggregation of platelets ex vivo. This study defined a concentration that reliably induces aggregation without overcoming inhibition by therapeutic aspirin therapy (ASA, 81‐mg) treatment. Logistic regression analysis of ex vivo aggregation, induced with increasing concentrations of AA in platelet‐rich plasma (PRP), estimated that platelets from ≥ 90% of subjects would aggregate at ≥ 1.5 mM AA (95% confidence interval [CI], 1.1, 2.1). A concentration of 1.6 mM AA failed to aggregate platelets from 26 healthy volunteers, who had previously aggregated at this concentration, following six daily oral doses of 81 mg of ASA. These data demonstrate that 1.6 mM AA reproducibly induces platelet aggregation in PRP from healthy volunteers without overcoming the antiplatelet effect of daily low‐dose aspirin therapy.