Life sciences (1973), 2020-02, Vol.242, p.117240
2020
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- Autor(en) / Beteiligte
- Titel
- Lycium barbarum polysaccharides attenuate kidney injury in septic rats by regulating Keap1-Nrf2/ARE pathway
- Ist Teil von
- Life sciences (1973), 2020-02, Vol.242, p.117240
- Ort / Verlag
- Netherlands: Elsevier BV
- Erscheinungsjahr
- 2020
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Lycium barbarum polysaccharides (LBP) are derived from Wolfberry and have antioxidant activities. This study aimed to evaluate the efficacy of LBP for kidney injury in a rat model of sepsis. Male rats were divided randomly to control group (Con), LPS group (LPS), ulinastatin group (ULI), low dose LBP group (LBP-1), middle dose LBP group (LBP-2) and high dose LBP group (LBP-3). After intraperitoneal injection of LPS (5 mg/kg) to make sepsis model (LPS group), 10,000 U/kg ulinastatin were given in ULI group, and 200, 400 and 800 mg/kg LBP was given in LBP-1, -2, -3 group, respectively. Serum IL-1β, IL-6, IL-8, TNF-α and NF-κB levels were measured by ELISA. Nrf2, Keap1, NF-κB, HO-1 and NQO1 expression levels were detected by PCR and Western blot analysis. We found that LBP decreased the levels of NF-κB and pro-inflammatory cytokines while attenuated kidney injury. In addition, LBP regulated Keap1-Nrf2/ARE signaling pathway in the kidney. In conclusion, LBP attenuates inflammation injury in the kidney via possible regulation of Keap1-Nrf2/ARE signaling.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0024-3205eISSN: 1879-0631DOI: 10.1016/j.lfs.2019.117240Titel-ID: cdi_proquest_journals_2350932055
- Format
- –
- Schlagworte
- Acute Kidney Injury - prevention & control, Animals, Antioxidant Response Elements - drug effects, Antioxidants, Attenuation, Blotting, Western, Cytokines, Cytokines - metabolism, Disease Models, Animal, Drugs, Chinese Herbal - pharmacology, IL-1β, Inflammation, Injuries, Interleukin 6, Interleukin 8, Kelch-Like ECH-Associated Protein 1 - metabolism, Kidneys, Lipopolysaccharides, Lycium barbarum, Male, NF-E2-Related Factor 2 - metabolism, NF-κB protein, Polysaccharides, Rats, Rats, Sprague-Dawley, Real-Time Polymerase Chain Reaction, Rodents, Saccharides, Sepsis, Sepsis - complications, Signal transduction, Signal Transduction - drug effects, Signaling, Tumor necrosis factor-α