FEMS microbiology letters, 1996-11, Vol.144 (2-3), p.171-176
1996
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Details
- Autor(en) / Beteiligte
- Titel
- Mapping of noninvasion TnphoA mutations on the Escherichia coli O18:K1:H7 chromosome
- Ist Teil von
- FEMS microbiology letters, 1996-11, Vol.144 (2-3), p.171-176
- Ort / Verlag
- Oxford, UK: Blackwell Publishing Ltd
- Erscheinungsjahr
- 1996
- Quelle
- Wiley Online Library - AutoHoldings Journals
- Beschreibungen/Notizen
- Abstract The most virulent newborn meningitis-associated Escherichia coli are of the serotype O18: K1: H7. We previously isolated a large number of E. coli O18:K1:H7 mutants resulting from transposon TnphoA mutagenesis that fail to invade brain microvascular endothelial cells. We have now determined the locations of 45 independent insertions. Twelve were localized to the 98 min region, containing a 120 kb segment that is characteristic of E. coli O18:K1:H7. Another, the previously described insertion ibe-10::TnphoA, was localized to the 87 min region, containing a 20 kb segment found in this E. coli. These noninvasion mutations may define new O18:K1:H7 pathogenicity islands carrying genes for penetration of the blood-brain barrier of newborn mammals.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0378-1097eISSN: 1574-6968DOI: 10.1111/j.1574-6968.1996.tb08526.xTitel-ID: cdi_proquest_journals_2331806957
- Format
- –
- Schlagworte
- Alkaline Phosphatase - genetics, Bacterial genomics, Bacterial Proteins - genetics, Bacteriology, Biological and medical sciences, Blood-Brain Barrier, Cells, Cultured, Chromosome Mapping, Chromosomes, Chromosomes, Bacterial - genetics, DNA Transposable Elements - genetics, E coli, Endothelial cells, Endothelium, Vascular - microbiology, Escherichia coli, Escherichia coli - classification, Escherichia coli - genetics, Escherichia coli - isolation & purification, Escherichia coli - pathogenicity, Escherichia coli Infections - microbiology, Escherichia coli meningitis, Fundamental and applied biological sciences. Psychology, Gene mapping, Genetics, Genome, Bacterial, Humans, Infant, Newborn, I‐SceI restriction endonuclease, Mapping, Meningitis, Meningitis, Bacterial - microbiology, Microbiology, Microvasculature, Mutagenesis, Mutagenesis, Insertional, Mutation, Pathogenicity, Pathogenicity island, Pathogenicity islands, Pathogens, Pulsed‐field gel electrophoresis, Rare‐restriction‐site mapping, Recombinant Fusion Proteins - genetics, Serotyping, Transposon TnphoA, Virulence - genetics