Details

Autor(en) / Beteiligte

• Bullo, Monica
• Peeraully, Muhammad R
• Trayhurn, Paul

Titel

Stimulation of NGF expression and secretion in 3T3-L1 adipocytes by prostaglandins PGD2, PGJ2, and 12-PGJ2

Ist Teil von

• American journal of physiology: endocrinology and metabolism, 2005-07, Vol.52 (1), p.E62

Ort / Verlag

Bethesda: American Physiological Society

Erscheinungsjahr

2005

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• Nerve growth factor (NGF) has recently been shown to be secreted from white adipocytes, its production being strongly stimulated by the proinflammatory cytokine tumor necrosis factor-{alpha}. In this study, we have examined whether a series of prostaglandins and other inflammation-related factors also stimulate NGF expression and secretion by adipocytes, using 3T3-L1 cells. Although interleukin (IL)-1{beta}, IL-10, and IL-18 each induced a small decrease in NGF mRNA level in 3T3-L1 adipocytes, there was no significant effect of these cytokines on NGF secretion. A small reduction in NGF expression and/or secretion was also observed with adiponectin and prostaglandins PGE2, PGF2{alpha}, and PGI2. In marked contrast, prostaglandin PGD2 induced a major, dose-dependent increase (up to 20- to 40-fold) in NGF expression and secretion. The PGD2 metabolites, PGJ2 and {Delta}12-PGJ2, also induced major increases (up to 30-fold) in NGF production. A further metabolite of PGJ2, 15-deoxy-{Delta}12,14-PGJ2, a peroxisome proliferator-activated receptor-{gamma} agonist, led paradoxically to a small increase in NGF mRNA level but a fall in NGF secretion. Both PGD2 and PGJ2 induced significant increases in NGF gene expression by 4 h after their addition. It is concluded that PGD2 and the J series prostaglandins, PGJ2 and {Delta}12-PGJ2, can play a significant role in the regulation of NGF production by white adipocytes. These results provide support for the view that NGF is an important inflammatory response protein, as well as a target-derived neurotrophin, in white adipose tissue. [PUBLICATION ABSTRACT]