Details

Autor(en) / Beteiligte

• BOES, Mary
• DAKE, Brian L
• BOOTH, Barbara A
• SANDRA, Alexander
• BATEMAN, Mathew
• KNUDTSON, Kevin L
• BAR, Robert S

Titel

IGF-I and IGFBP-3 transport in the rat heart

Ist Teil von

• American journal of physiology: endocrinology and metabolism, 2003, Vol.47 (1), p.237-239

Ort / Verlag

Bethesda, MD: American Physiological Society

Erscheinungsjahr

2003

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• Specific binding of IGF-binding protein (IGFBP)-3 was shown to be present in the isolated, beating rat heart. The uptake of perfused 125I-labeled IGF-I in the beating heart was decreased to 9% by blocking IGF-I binding sites with the IGF-I analog Long R3 (LR3) IGF-I. When LR3 was perfused with complexes of 125I-IGF-I x IGFBP-3, uptake of 125I-IGF-I was decreased to 41%, which was significantly greater than LR3 and 125I-IGF-I (41 vs. 9%). These data suggest that both microvessel IGF-I and IGFBP-3 binding sites contribute to the transport of IGF-I in the perfused rat heart. This also suggests a novel and plausible mechanism whereby circulating IGFs reach sites of IGF bioactivity.