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Development of a selective medium and antisera for Pseudomonas syringae pv. syringae from seeds of barley and wheat
Ist Teil von
Journal of general plant pathology : JGPP, 2019-05, Vol.85 (3), p.211-220
Ort / Verlag
Tokyo: Springer Japan
Erscheinungsjahr
2019
Link zum Volltext
Quelle
SpringerLink (Online service)
Beschreibungen/Notizen
Bacterial black node, a disease of barley and wheat, is caused by
Pseudomonas syringae
pv.
syringae
(synonym pv.
japonica
, hereafter
Psj
). Here a selective medium, serine-potassium tellurite-based Psj-selective agar (SPTPsjA) was developed to isolate and detect
Psj
. After 5–7 days on SPTPsjA at 25 °C, characteristic black
Psj
colonies formed with an efficiency equal to that on potato–peptone–glucose agar. Except for
P. viridiflava, P. cichorii
, and some strains of
P. syringae
group bacteria, SPTPsjA either inhibited growth of other phytopathogenic bacteria or enabled their discrimination. To facilitate diagnosis of
Psj
, we prepared three antisera against three strains; in a slide agglutination test, the antisera reacted to all tested strains of
Psj
and failed to react to
P. viridiflava
and
P. cichorii
although some strains of other
P. syringae
group bacteria were indistinguishable from
Psj
with SPTPsjA and antisera. When barley and wheat seeds from fields in which bacterial black node had occurred were placed on SPTPsjA in the dark at 25 °C for 7 days, black colonies grew on the medium around the seeds. Among these strains, only those responsive to the above antisera were pathogenic on barley. These results show that the combination of SPTPsjA and antisera can isolate
Psj
from seeds of barley and wheat.