Details

Autor(en) / Beteiligte

• Li, I. A
• Popov, A. M
• Tsybul'skii, A. V
• Sanina, N. M
• Kostetskii, E. Ya
• Novikova, O. D
• Portnyagina, O. Yu
• Mazeika, A. V

Titel

Immunostimulatory characteristics of a novel adjuvant on the basis of cucumarioside A₂-2 and monogalactosyldiacylgycerol

Ist Teil von

• Applied biochemistry and microbiology, 2008-11, Vol.44 (6), p.629-634

Ort / Verlag

Dordrecht: Dordrecht : SP MAIK Nauka/Interperiodica

Erscheinungsjahr

2008

Quelle

SpringerLink

Beschreibungen/Notizen

• A novel antigen carrier has been formulated on the basis of a cucumarioside-A₂-2 triterpene glycoside (CD) complex with cholesterol and monogalactosyldiacylglycerol from Ahnfeltia tobuchiensis (MGDGAt) and Ulva fenestrate (MGDGUf). Morphological and immunostimulative characteristics of the carrier were studied. Electron microscopy experiments demonstrated the formation of homogeneous tubular structures in a mixture of CD, cholesterol, and MGDG in molar ratio of 1: 2: 3. In animals immunized by the carrier bearing pore forming protein monomer of pseudotuberculosis agent CD and MGDG synergically affected synthesis of specific antibodies, interleukin-2, and γ-interferon and delayed hypersensitivity reaction when compared to Freund's complete adjuvant or to immunostimulatory complexes between Quillaja saponaria saponins and phosphatidylcholine from egg yolk. The immunostimulatory effect depends upon the composition of polyunsaturated fatty acids of MGDG. The new tubular adjuvant carrier is a competitive adjuvant, as it includes CD obtained from far-eastern sea cucumber commercial species Cucumaria japonica, and MGDG from seaweed.