Details

Autor(en) / Beteiligte

• BYOUNG YWONG KIM
• CHO, Chi-Heum
• BAE, Insoo
• JAE HOON BAE
• SONG, Dae-Kyu
• MUN, Kyo-Cheol
• SUH, Seong-Il
• KIM, Sang-Pyo
• SHIN, Dong-Hoon
• JANG, Byeong-Churl
• TAEG KYU KWON
• CHA, Soon-Do

Titel

Ciglitizone inhibits cell proliferation in human uterine leiomyoma via activation of store-operated Ca2+ channels

Ist Teil von

• American Journal of Physiology: Cell Physiology, 2005-02, Vol.57 (2), p.C389-C395

Ort / Verlag

Bethesda, MD: American Physiological Society

Erscheinungsjahr

2005

Link zum Volltext

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• This study investigated the acute effects of a peroxisome proliferator-activated receptor (PPAR)-{gamma} ligand, ciglitizone, on cell proliferation and intracellular Ca2+ signaling in human normal myometrium and uterine leiomyoma. Changes in intracellular Ca2+ concentration ([Ca2+]i) were measured with fura-2 AM, and cellular viabilities were determined by viable cell count and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide reduction assay. Ciglitizone (100 microM) induced greater inhibition of cell proliferation in uterine leiomyoma than in myometrium. Ciglitizone also dose-dependently increased [Ca2+]i in both myometrium and uterine leiomyoma; these [Ca2+]i increases were inhibited by PPAR-{gamma} antagonists and raloxifene. Ciglitizone-induced [Ca2+]i increase showed only an initial peak in normal myometrial cells, whereas in uterine leiomyoma there was a second sustained [Ca2+]i increase as well. The initial [Ca2+]i increase in both myometrium and uterine leiomyoma resulted from the release of Ca2+ by the sarcoplasmic reticulum via activation of ryanodine receptors. The second [Ca2+]i increase was observed only in uterine leiomyoma because of a Ca2+ influx via an activation of store-operated Ca2+ channels (SOCCs). Cell proliferation was inhibited and secondary [Ca2+]i increase in uterine leiomyoma was attenuated by cotreatment of ciglitizone with a SOCC blocker, lanthanum. The results suggest that ciglitizone inhibits cell proliferation and increases [Ca2+]i through the activation of SOCCs, especially in human uterine leiomyoma. [PUBLICATION ABSTRACT]