Es ist ein Fehler in der Kommunikation mit einem externen System aufgetreten. Bitte versuchen Sie Ihre letzte Aktion erneut. Sollte der Fehler bestehen bleiben, setzen Sie sich bitte mit dem Informationszentrum der Bibliothek in Verbindung oder versuchen Sie es später erneut.
American Journal of Physiology: Cell Physiology, 2009-02, Vol.296 (2), p.C256-C266
2009
Volltextzugriff (PDF)
Details
- Autor(en) / Beteiligte
- Titel
- AIF-1 expression regulates endothelial cell activation, signal transduction, and vasculogenesis
- Ist Teil von
- American Journal of Physiology: Cell Physiology, 2009-02, Vol.296 (2), p.C256-C266
- Ort / Verlag
- United States: American Physiological Society
- Erscheinungsjahr
- 2009
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Department of Physiology, Independence Blue Cross Cardiovascular Research Center, Temple University School of Medicine, Philadelphia, Pennsylvania Submitted 19 June 2008 ; accepted in final form 8 September 2008 Endothelial cell (EC) activation plays a key role in vascular inflammation, thrombosis, and angiogenesis. Allograft inflammatory factor-1 (AIF-1) is a cytoplasmic, calcium-binding, inflammation-responsive scaffold protein that has been implicated in the regulation of inflammation. The expression and function of AIF-1 in EC is uncharacterized, and the purpose of this study was to characterize AIF-1 expression and function in ECs. AIF-1 expression colocalized with CD31-positive ECs in neointima of inflamed human arteries but not normal arteries. AIF-1 is detected at low levels in unstimulated EC, but expression can be increased in response to serum and soluble factors. Stable transfection of AIF-1 small interfering RNA (siRNA) in ECs reduced AIF-1 protein expression by 73% and significantly reduced EC proliferation and migration ( P < 0.05 and 0.001). Rescue of AIF-1 expression restored both proliferation and migration of siRNA-expressing ECs, and AIF-1 overexpression enhanced both of these activities, suggesting a strong association between AIF-1 expression and EC activation. Activation of mitogen-activated protein kinase p44/42 and PAK1 was significantly reduced in siRNA ECs challenged with inflammatory stimuli. Reduction of AIF-1 expression did not decrease EC tube-like structure or microvessel formation from aortic rings, but overexpression of AIF-1 did significantly increase the number and complexity of these structures. These data indicate that AIF-1 expression plays an important role in signal transduction and activation of ECs and may also participate in new vessel formation. small interfering RNA; vascular inflammation; allograft inflammatory factor-1 Address for reprint requests and other correspondence: M. Autieri, Temple Univ. School of Medicine, Rm. 810, MRB, 3420 N. Broad St., Philadelphia, PA 19140 ( mautieri{at}temple.edu )
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0363-6143eISSN: 1522-1563DOI: 10.1152/ajpcell.00325.2008Titel-ID: cdi_proquest_journals_230337136
- Format
- –
- Schlagworte
- Animals, Aorta, Thoracic - metabolism, Cattle, Cell Movement, Cell Proliferation, Cells, Cells, Cultured, Coronary Vessels - metabolism, DNA-Binding Proteins - metabolism, Endothelial Cells - enzymology, Endothelial Cells - metabolism, Enzyme Activation, Female, Gene expression, Humans, Inflammation - metabolism, Inflammation Mediators - metabolism, Kinases, Male, Mice, Mice, Inbred C57BL, Mitogen-Activated Protein Kinase 1 - metabolism, Mitogen-Activated Protein Kinase 3 - metabolism, Neovascularization, Physiologic, p21-Activated Kinases - metabolism, Ribonucleic acid, RNA, RNA Interference, Signal Transduction, Studies, Time Factors, Tissue Culture Techniques, Transfection, Vascular Biology, Veins & arteries