Journal of dental research, 2012-04, Vol.91 (4), p.407-412
2012
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Details
- Autor(en) / Beteiligte
- Titel
- Effects of Recombinant Dentin Sialoprotein in Dental Pulp Cells
- Ist Teil von
- Journal of dental research, 2012-04, Vol.91 (4), p.407-412
- Ort / Verlag
- Los Angeles, CA: SAGE Publications
- Erscheinungsjahr
- 2012
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Dentin sialophosphoprotein (DSPP) is critical for dentin mineralization. However, the function of dentin sialoprotein (DSP), the cleaved product of DSPP, remains unclear. This study aimed to investigate the signal transduction pathways and effects of recombinant human DSP (rh-DSP) on proliferation, migration, and odontoblastic differentiation in human dental pulp cells (HDPCs). The exogenous addition of rh-DSP enhanced the proliferation and migration of HDPCs in dose- and time-dependent manners. rh-DSP markedly increased ALP activity, calcium nodule formation, and levels of odontoblastic marker mRNA. rh-DSP increased BMP-2 expression and Smad1/5/8 phosphorylation, which was blocked by the BMP antagonist, noggin. Furthermore, rh-DSP phosphorylated extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK), Akt, and IκB-α, and induced the nuclear translocation of the NF-κB p65 subunit. Analysis of these data demonstrates a novel signaling function of rh-DSP for the promotion of growth, migration, and differentiation in HDPCS via the BMP/Smad, JNK, ERK, MAPK, and NF-κB signaling pathways, suggesting that rh-DSP may have therapeutic utility in dentin regeneration or dental pulp tissue engineering.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0022-0345eISSN: 1544-0591DOI: 10.1177/0022034511436113Titel-ID: cdi_proquest_journals_2300580662
- Format
- –
- Schlagworte
- AKT protein, Alkaline Phosphatase - drug effects, Bone Morphogenetic Protein 2 - drug effects, Bone Morphogenetic Proteins - antagonists & inhibitors, c-Jun protein, Calcium, Carrier Proteins - pharmacology, Cell adhesion & migration, Cell differentiation, Cell Differentiation - drug effects, Cell growth, Cell Line, Cell Movement - drug effects, Cell proliferation, Cell Proliferation - drug effects, Dental pulp, Dental Pulp - cytology, Dental Pulp - drug effects, Dentin, Dentin sialoprotein, Dose-Response Relationship, Drug, Dspp protein, Extracellular Matrix Proteins - pharmacology, Extracellular signal-regulated kinase, Gene expression, Genetic disorders, Humans, I-kappa B Proteins - drug effects, JNK Mitogen-Activated Protein Kinases - drug effects, JNK protein, Kinases, MAP kinase, Mineralization, mRNA, Mutation, NF-kappa B - antagonists & inhibitors, NF-KappaB Inhibitor alpha, NF-κB protein, Nodules, Noggin protein, Nuclear transport, Odontoblasts - drug effects, Oncogene Protein v-akt - drug effects, Phosphoproteins - pharmacology, Phosphorylation, Proteins, Recombinant Proteins, RNA, Messenger - drug effects, Sialoglycoproteins - pharmacology, Signal transduction, Signal Transduction - drug effects, Smad protein, Smad1 Protein - drug effects, Smad5 Protein - drug effects, Smad8 Protein - drug effects, Stem cells, Time Factors, Tissue engineering, Tooth Calcification - drug effects, Transcription Factor RelA - drug effects, Transcription factors, Transforming Growth Factor beta - drug effects