Details

Autor(en) / Beteiligte

• Morikawa, Miki
• Inoue, Yoshiaki
• Sumi, Yuka
• Kuroda, Yoko
• Tanaka, Hiroshi

Titel

Leukocyte deformability in sepsis‐induced DIC

Ist Teil von

• Acute medicine & surgery, 2015-01, Vol.2 (1), p.13-20

Ort / Verlag

Hoboken: John Wiley & Sons, Inc

Erscheinungsjahr

2015

Quelle

Wiley Online Library All Journals

Beschreibungen/Notizen

• AimSepsis‐induced disseminated intravascular coagulopathy is associated with a high mortality rate. The function and deformability of polymorphonuclear leukocytes change in patients with sepsis. The goal of this study was to characterize the changes in polymorphonuclear leukocyte deformability in patients with sepsis‐induced disseminated intravascular coagulopathy and to evaluate the relationship between the severity of disseminated intravascular coagulopathy and the deformability of polymorphonuclear leukocytes.MethodsThirty‐five patients with sepsis‐induced disseminated intravascular coagulopathy at our department were enrolled in this study. These patients were diagnosed with severe sepsis and an acute disseminated intravascular coagulopathy score ≥ 4. Blood samples were obtained from these patients on days 1, 3, and 7. Polymorphonuclear leukocyte deformability was measured with a microchannel flow analyzer, and polymorphonuclear leukocyte activity, represented as CD11b, was measured by flow cytometry. In contrast, 14 patients who fulfilled with sepsis criteria but without complicated disseminated intravascular coagulopathy were also entered in this study.ResultsIn patients with sepsis‐induced disseminated intravascular coagulopathy, there was a significant correlation between their Japanese Association for Acute Medicine disseminated intravascular coagulopathy score and polymorphonuclear leukocyte deformability, and CD11b expression. Polymorphonuclear leukocytes became more stiffened and CD11b expression was higher in patients with sepsis‐induced disseminated intravascular coagulopathy compared to patients without the condition.ConclusionPolymorphonuclear leukocyte deformability correlated with the severity of sepsis‐induced disseminated intravascular coagulopathy and the response to treatment.