Details

Autor(en) / Beteiligte

• Pogozhykh, D
• Prokopyuk, V
• Pogozhykh, O
• Goltsev, A
• Blasczyk, R
• Börgel, M
• Figueiredo, C

Titel

LOW TEMPERATURE STORAGE OF NATURAL AND BIOENGINEERED MULTICELLULAR PLACENTAL CONSTRUCTS

Ist Teil von

• International journal of artificial organs, 2019-08, Vol.42 (8)

Ort / Verlag

Milan: Wichtig Editore s.r.l

Erscheinungsjahr

2019

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Placental complex, including placental tissues, foetal membranes, and umbilical cord, is one of the most promising sources of a range of tissues and cells for clinical and experimental application in regenerative medicine and tissue engineering. Certain components of placental material are already applied in medicine since decades, while others undergo intensive research and pre-clinical trials. Since placenta is a temporary organ, it provides the unique opportunity to obtain large amounts of tissues, including autologous, without the adverse effects to the donor. Moreover, great potential of placental multipotent stromal cells is recognized in generation of tissue engineered multicellular constructs. At the same time, clinical use and research proceedings are significantly restricted due to imperfections in storage technologies. Application of low-temperature preservation methods is especially challenging for 3-D multicellular structures, such as organs, tissues, and tissue engineered constructs. Here we aim to analyze the variety of cryoinjuries in a range of placental tissues and tissue engineered multicellular placental constructs, as well as support the cellular viability after low temperature storage. A range of cell and tissue culture as well as tissue engineering methods, cryomicroscopic analysis, evaluation of phenotypic and metabolic characteristics have been applied in this study. Results have shown that the mechanisms of damage caused by cryopreservation procedures depend on particular structure of the studied object, peculiarities of intercellular bonds, as well as interaction with cryoprotective agents. At the same time, developed protocols allowed us to efficiently isolate viable cells after thawing of the studied tissues. Conclusions of this study may lead to progress in improvement of biobanking technologies for tissues and tissue engineered multicellular constructs.