Details

Autor(en) / Beteiligte

• Cai-cai, BAO
• Bo-yao, YUAN
• Meng-jiao, SUN
• Man-xia, WANG
• QI, Fei
• Yan-xia, NIU

Titel

Autophagy regulation and anti-inflammatory effect of curcumin on the mouse with experimental autoimmune encephalomyelitis

Ist Teil von

• Jie fang jun yi xue za zhi, 2019-07, Vol.44 (7), p.593

Ort / Verlag

Beijing: People's Military Medical Press

Erscheinungsjahr

2019

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• Objective 　To investigate the anti-inflammatory effect of curcumin on the mouse with experimental autoimmune encephalomyelitis (EAE), and explore its mechanism of action. Methods 　Forty female C57BL/6 mice were randomly divided into EAE+DMSO group, EAE+curcumin group, DMSO group and curcumin group with10 mice in each group. Mice in EAE+DMSO group and EAE+curcumin group were used to establish the EAE model. DMSO group was the solvent control of EAE+DMSO group, and 0.2 ml 20% DMSO solution daily were intraperitoneally injected in the both groups a week after immunization; and mice in curcumin group and EAE+curcumin group were daily intraperitoneally injected with 10 mg/kg curcumin solution a week after immunization. From the date of immunization, the mice in each group were evaluated for clinical symptoms every day. Mice were anesthetized and sacrificed at the peak period of EAE (21 d), their spinal cord tissues were taken for HE staining, serum concentrations of INF-γ and IL-17 were measured by ELISA, spleen l