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Heterogeneous expression of the cotton R2R3-MYB transcription factor GbMYB60 increases salt sensitivity in transgenic Arabidopsis
Ist Teil von
Plant cell, tissue and organ culture, 2018-04, Vol.133 (1), p.15-25
Ort / Verlag
Dordrecht: Springer Netherlands
Erscheinungsjahr
2018
Quelle
SpringerLink
Beschreibungen/Notizen
MYB proteins form one of the largest transcription factor families in plants. Growing evidence has suggested that MYB has important roles in the regulation of plant growth and development, as well as responses to abiotic and biotic stresses. In the present study, the
GbMYB60
gene isolated from
Gossypium barbadense
was heterogeneously expressed in
Arabidopsis. GbMYB60
encodes a R2R3-MYB protein with transcriptional activation activity. Histochemical staining revealed that
GbMYB60
was preferential expressed in the leaf vascular and meristem tissues of young seedlings and in the axillary bud tissue of mature plants. Expression pattern analysis indicated that
GbMYB60
is induced by ABA, mannitol, and salt, but not H
2
O
2
. Transgenic
Arabidopsis
with constitutively higher expression of
GbMYB60
exhibited reduced germination rates under salt and mannitol treatments as well as reduced tolerance to salt at the adult-plant stage. Transgenic
Arabidopsis
accumulates more reactive oxygen species compared to wild-type plants under stress conditions, corresponding with more severe cell death in transgenic plants. Additionally, ABA-related genes, including
ABI5
and
ABF3
, were down-regulated in
GbMYB60
-overexpressing plants. This research has revealed that cotton GbMYB60 negatively regulates plant tolerance to salt stress, possibly through the inhibition of ABA signaling pathways and enhancing reactive oxygen species over-production.