Details

Autor(en) / Beteiligte

• Roth‐Konforti, Michal
• Green, Ori
• Hupfeld, Mario
• Fieseler, Lars
• Heinrich, Nadine
• Ihssen, Julian
• Vorberg, Raffael
• Wick, Lukas
• Spitz, Urs
• Shabat, Doron

Titel

Ultrasensitive Detection of Salmonella and Listeria monocytogenes by Small‐Molecule Chemiluminescence Probes

Ist Teil von

• Angewandte Chemie, 2019-07, Vol.131 (30), p.10469-10475

Ort / Verlag

Weinheim: Wiley Subscription Services, Inc

Erscheinungsjahr

2019

Quelle

Wiley-Blackwell Journals

Beschreibungen/Notizen

• Detection of Salmonella and L. monocytogenes in food samples by current diagnostic methods requires relatively long time to results (2–6 days). Furthermore, the ability to perform environmental monitoring at the factory site for these pathogens is limited due to the need for laboratory facilities. Herein, we report new chemiluminescence probes for the ultrasensitive direct detection of viable pathogenic bacteria. The probes are composed of a bright phenoxy‐dioxetane luminophore masked by triggering group, which is activated by a specific bacterial enzyme, and could detect their corresponding bacteria with an LOD value of about 600‐fold lower than that of fluorescent probes. Moreover, we were able to detect a minimum of 10 Salmonella cells within 6 h incubation. The assay allows for bacterial enrichment and detection in one test tube without further sample preparation. We anticipate that this design strategy will be used to prepare analogous chemiluminescence probes for other enzymes relevant to specific bacteria detection and point‐of‐care diagnostics. Salmon und Lister wären stolz: Die Entwicklung neuartiger Chemilumineszenzsonden für die direkte Detektion zwei der weitverbreitesten und tödlichsten Bakterien in Lebensmittelvergiftungen, Salmonella und Listeria monocytogenes, wird beschrieben. Die zwei Sonden können die Bakterien mit einer Nachweisgrenze detektieren, die ca. 600‐mal niedriger ist als die von Fluoreszenzsonden.